cobe 2991 cell processor (Terumo BCT)

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Terumo BCT cobe 2991 cell processor
Cobe 2991 Cell Processor, supplied by Terumo BCT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cobe+2991+cell+processor/cobe+2991+cell+processor/pmc11338550-68-13-17
Average 90 stars, based on 1 article reviews

cobe 2991 cell processor - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Metabolic measures before surgery and long-term diabetes outcomes in recipients of total pancreatectomy and islet autotransplantation
Article Snippet: Purification with density centrifugation (COBE 2991 cell processor; Terumo BCT, Lakewood, CO) was performed if needed to reduce the tissue volume (generally performed for tissue volumes >0.25 ml/kg).

Isolation:

Article Title: Utility of Islet Cell Preparations From Donor Pancreases After Euthanasia.
Article Snippet: .. Islet cells were isolated using a modification of the automated Ricordi method17 and purified by continuous gradient with Biocoll (Biochrom, Berlin, Germany) and a cooled COBE 2991 cell processor (Terumo BCT, Lakewood, CO, USA). .. After isolation and purification, the cell preparations were cultured in cell culture flasks T175 (Sarstedt, Nümbrecht, Germany) at 37°C in a humidified incubator (5% CO2) in a Ham’s F10 based medium (Lonza, Bazel, Switzerland).

Modification:

Purification:

Article Title: Which detergent is most suitable for the generation of an acellular pancreas bioscaffold?
Article Snippet: Pancreatic islets were isolated after ductal distension of the pancreas and tissue digestion with collagenase and neutral protease (SERVA Electrophoresis, Germany), according to the automated method of Ricordi (27) with modifications (28), as previously described (29). .. Briefly, islet purification was achieved using a continuous Ficoll density gradient in a COBE 2991 Cell Processor (Gambro/Terumo BCT, Japan). .. The islet preparation used in this study exhibited a 70±4% purity, as determined by dithizone staining, and greater than 80% islet cell viability, which was evaluated using the live/dead fluorescent method, based on incorporation of either fluorescein diacetate (Sigma-Aldrich, USA) by live cells or propidium iodide (Sigma-Aldrich, USA) by dead cells.

Article Title: Which detergent is most suitable for the generation of an acellular pancreas bioscaffold?
Article Snippet: Pancreatic islets were isolated after ductal distension of the pancreas and tissue digestion with collagenase and neutral protease (SERVA Electrophoresis, Germany), according to the automated method of Ricordi ( ) with modifications , as previously described ( ). .. Briefly, islet purification was achieved using a continuous Ficoll density gradient in a COBE 2991 Cell Processor (Gambro/Terumo BCT, Japan). .. The islet preparation used in this study exhibited a 70±4% purity, as determined by dithizone staining, and greater than 80% islet cell viability, which was evaluated using the live/dead fluorescent method, based on incorporation of either fluorescein diacetate (Sigma-Aldrich, USA) by live cells or propidium iodide (Sigma-Aldrich, USA) by dead cells.

Article Title: Early Metabolic Measures Predict Long-term Insulin Independence in Recipients of Total Pancreatectomy and Islet Autotransplantation
Article Snippet: .. After digestion, the islets were purified using density gradients in a COBE 2991 cell processor (Terumo BCT, Lakewood, CO) if needed to reduce transplanted tissue volume (generally performed for a postdigest tissue volume >0.25 mL/kg). ..

Centrifugation:

Article Title: A transcriptional cross species map of pancreatic islet cells
Article Snippet: .. Islets were separated from exocrine tissue by centrifugation on a discontinuous Ficoll (Sigma–Aldrich, Taufkirchen, Germany) density gradient in a COBE 2991 cell processor (Terumo BCT). .. After purification, islets were cultured in CMRL 1066 medium supplemented with 10% heat inactivated FBS, 100U/mL penicillin, 0.1 mg/ml streptomycin (all Gibco®, Darmstadt, Germany) and 32.5 mM l -glutathione (Sigma–Aldrich, Taufkirchen, Germany) at 37 °C in a 5% CO 2 incubator.

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